Abstract:
Introduction: The current study was conducted to evaluate
the impact of low-intensity eccentric resistance training
combined with and without blood flow restriction (ECCRT
with BFR vs. ECCRT without BFR) on some of the
systematic and genetic indices affecting the activation and
proliferation of satellite cells in young non-athlete men.
Materials and Methods: Twenty men with an age range of
25±5 years were randomly divided into two groups of
ECCRT with BFR and ECCRT without BFR (each group
containing 10 subjects). ECCRT was performed using
isokinetic device. It involves about 70 rpt in knee extensor
muscles. Restriction was created using an air pressure meter.
After local anesthesia through injecting lidocaine 1% and
norepinephrine, sampling was performed from lateral broad
muscle 48 hours before and 24 hours after the training, and
the systematic and genetic indices affecting the activation
and proliferation of satellite cells (HGF and Myf5) were
examined and compared in two stages of pre-test and posttest
in two situations of ECCRT with BFR vs. ECCRT
without BFR.
Results: The results of this study showed that HGF and
Myf5 increased after ECCRT with BFR and ECCRT
without BFR (P<0.05) and the increase of HGF and Myf5
was higher after ECCRT with BFR than after ECCRT
without BFR (P<0.05).
Conclusions: It is recommended for authorities and officials
in the sports field to use low-intensity ECCRT with BFR to
increase HGF and Myof5.
Machine summary:
Evaluating the effect of low-intensity eccentric resistance training combined with blood flow restriction on the systematic and genetic indices affecting the activation and proliferation of satellite cells in young non-athlete men Azam Mousavian1, Abbas Ali Gaeni2, Reza Nuri*3, Mohammad Reza Kordi2 Received: 26 Jun 2017/ Accepted: 10 August 2017 (1) PhD Student in Exercise Physiology, Kish international campus, University of Tehran, Iran (2)Department of Exercise Physiology, Faculty of Physical Education and Sport Science, University of Tehran, Tehran, Iran.
After local anesthesia through injecting lidocaine 1% and norepinephrine, sampling was performed from lateral broad muscle 48 hours before and 24 hours after the training, and the systematic and genetic indices affecting the activation and proliferation of satellite cells (HGF and Myf5) were examined and compared in two stages of pre-test and post- test in two situations of ECCRT with BFR vs.
(2004) reported an increase in the number of satellite cells after high-intensity eccentric resistance training (ECCRT) of leg extensor muscles in eight healthy men, by using isokinetic dynamometer (8).
(2012) also reported an increase in the number of satellite cells along with increase in muscle strength and muscle hypertrophy as a result of three weeks of low-intensity resistance training (20% of 1RM) on the knee extensor muscles of 20 healthy men (using isokinetic dynamometer) combined with BFR (9).
Discussion In the present study, ECCRT with BFR caused an increase in level of HGF and Myf5 gene expression, suggesting the effect of this training on the activation and proliferation of satellite cells.
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