چکیده:
To study this, a research was conducted in greenhouse in a factorial experiment based on randomized complete blocks design with three replications. The first factor included stress levels and the second factor included genotypes. To apply Drought stress metanol was used. Seeds were planted in plastic pots in greenhouse conditions and plants irrigation was done by Hoagland nutrient solution. The proline and chlorophyll of shoots were measured by the end of growth stage. Results suggested that genotype No. 4 with a mean of 19.25 had the highest rate of chlorophyll and genotype No. 1 with mean of 13.25 had the lowest chlorophyll rate. Also, genotype No. 3 with a mean of 5.86 had the highest proline rate. Results indicated that Drought stress application could decrease chlorophyll rate while it increases proline rate. Decrease in plant chlorophyll decreases the photosynthetic activity. Increase in proline along with increase in Drought level specifies the osmotic balance maintenance in low water potential. Results generally showed that increase in proline production as an osmotic regulatory mechanism in high Drought levels which decreases the seedling growth.
خلاصه ماشینی:
Effects of osmotic stress on chlorophyll and proline different wheat cultivars Bahram Mirzamasoumzadeh1*and vahid Mollasadeghi2 1Young Researchers Club, Science and Research Branch, Islamic Azad University, Ardabil, Iran 2 Department of Agronomy and Plant Breeding, Ardabil branch, Islamic Azad University, Ardabil, Iran *Corresponding author’s E-mail: bm_masoumzadeh@yahoo.
Results indicated that Drought stress application could decrease chlorophyll rate while it increases proline rate.
Results generally showed that increase in proline production as an osmotic regulatory mechanism in high Drought levels which decreases the seedling growth.
Introduction Increase in the world population has added to the significance of considering the reduction of freshwater resources, agricultural lands salinization and the feasibility of tolerant plants in unfavorable environmental condition.
Increase in Drought level increases the osmotic regulator amount (proline) which could result in plant tolerance towards environmental stress [10].
Iranian Journal of Research in Science and Engineering aluminum sheets and were put in liquid nitrogen at -80 °C, Table(2): Studied Traits ANOVAwas pounded in pounder and homogenized by sulfosalicylic acid (3 percent) Homogenized solutions were centrifuged for 20 minutes at 4 °C in 5000 rmp, by refrigerated centrifuge KUBOTA 6900 (Made in Japan).
01, respectively and ns No significant difference Table (3): Mean Comparison Table for Various Drought levels on Studied Traits Characters Stress Levels Chlorophyll Proline water (control) 18.
Proline is the most effective osmotic regulator substance in plants affected by Drought [14].
Accumulation of proline and pattern of amino acids in sugar wheat plants in response to osmotic, water and salt stress.