خلاصة:
Calcineurin, which functions in calcium signaling, is expressed in skeletal and cardiac muscle and has been linked to sensitivity to muscle strength training. It is also proposed to contribute to individual aerobic endurance. To investigate the relationship between calcineurin-encoding genes and aerobic endurance traits, 126 young-adult Han Chinese males were enrolled in an aerobic exercise training study. Participants were genotyped for polymorphisms within the 5 genes (PPP3CA, PPP3CB, PPP3CC, PPP3R1 and PPP3R2) encoding calcineurin using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) or matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Participants underwent 18 weeks of aerobic exercise training (running). Before and after the training period, maximal oxygen uptake (VO2max) and 12 km/h running economy were measured. Statistical analyses were performed using chi-square test and analysis of variance. The baseline value of VO2max was significantly associated with rs3804423 and rs2850965 loci in the PPP3CA gene (P<0.05). The training responsiveness of VO2max was significantly associated with the rs3804358 locus in PPP3CA and the rs4671887 locus in PPP3R1 (P<0.05). The training responsiveness of running economy was significantly associated with rs3739723 in PPP3R2 (P<0.05). These findings indicate that polymorphisms within the calcineurin-encoding genes may partially explain individual differences in response to aerobic training.
ملخص الجهاز:
"Single Nucleotide Polymorphisms within Calcineurin-Encoding Genes are Associated with Response to Aerobic Training in Han Chinese Males 1Rong-mei Xu, 2Tao Lu, 2Lingxian Yan, 1Qinghua Song* 1The Center of Physical Health, Henan Polytechnic University, Jiaozuo 454000, Henan Province, China.
To investigate the relationship between calcineurin-encoding genes and aerobic endurance traits, 126 young-adult Han Chinese males were enrolled in an aerobic exercise training study.
Participants were genotyped for polymorphisms within the 5 genes (PPP3CA, PPP3CB, PPP3CC, PPP3R1 and PPP3R2) encoding calcineurin using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) or matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Given its interaction with calcium, presence in muscle tissues, and fluctuations during exercise, CN is proposed to correlate with individual differences in response to aerobic training.
In this study, single nucleotide polymorphisms (SNPs) within the five genes encoding CN, PPP3CA, PPP3CB, PPP3CC, PPP3R1 and PPP3R2, were investigated for a potential role in aerobic training response.
Specifically, genotypes were compared against maximal oxygen uptake (VO2max) and running economy (RE) to explore the correlation between CN variants and individual differences in response to aerobic training.
RESULTS To determine whether variants in the genes encoding calcineurin are associated with response to aerobic training, genotypes of participants were compared with various exercise measures following an 18-week aerobic training (Table 2).
rs3739723 in the PPP3R2 gene was found in this study to be correlated with the sensitivity to training for running economy, which is a determining factor for aerobic capacity under submaximal load (19)."