Abstract:
Background. Desnutrin is an enzyme that catalyzes the first step of cytoplasmic triacylglycerol lipolysis from white adipose tissue and several other tissues, which are disrupted by the obesity and metabolic syndrome.
Objectives. The aim of this study was to compare the effect of high-intensity interval training and continuous training on the desnutrin gene expression in the subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats.
Methods. For this purpose, after 12 weeks on a high-fat diet, 30 Wistar rats were divided into two groups: continuous training (n =10) and high-intensity interval training (n=10). Continuous (80%VO2max) and high-intensity interval exercise training (100%VO2max) sessions were conducted according to the principle of overload for six weeks and six sessions per week. Forty-eight hours after the last training session, the abdominal subcutaneous adipose tissue and quadriceps muscle tissue were extracted, and the expression level was assessed using the RT-PCR method.
Results. The study showed that the difference between high-intensity interval and continuous training on the desnutrin gene expression subcutaneous adipose tissue was significant (p=0.004), but there were no significant differences (p=0.415) between them in the quadriceps femoris muscle tissue.
Conclusion. According to the findings of the present study, in the adipose tissue, continuous training has a greater effect on the desnutrin gene expression than high-intensity interval training. In the muscle tissue, however, there is no difference between these two training exercises
Machine summary:
The Effect of High-Intensity Interval Training andContinuous Training on the Desnutrin Gene Expression in theSubcutaneous Adipose Tissue and the Quadriceps FemorisMuscle Tissue of Obese Male Rats1Saeed Rahmaty*, 1Abbas Ali Gaeini, 2Mojtaba Dolatshahi, 1Siroos Choobineh1Department of Exercise Physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran.
The aimof this study was to compare the effect of high-intensity interval training and continuous training on the desnutrin geneexpression in the subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats.
Forty-eight hours after the last training session, the abdominal subcutaneous adipose tissue and quadriceps muscle tissuewere extracted, and the expression level was assessed using the RT-PCR method.
The study showed that thedifference between high-intensity interval and continuous training on the desnutrin gene expression subcutaneous adiposetissue was significant (p=0.
According to the findings of the present study, in the adipose tissue, continuoustraining has a greater effect on the desnutrin gene expression than high-intensity interval training.
(2015) showedthat six weeks and three sessions of high-intensityinterval training (HIIT) per week have no effect onthe level of ATGL and mitochondrial fat oxidationin muscle tissue and the subcutaneous fatty tissueof inactive overweight human subjects (7).
Therefore, the aim of this study was to comparethe effect of six weeks of continuous training withhigh-intensity interval training on the desnutringene expression of the subcutaneous adiposetissue and the quadriceps femoris muscle tissue ofobese male rats.
Inthis study, high-intensity interval trainingslightly increased the desnutrin geneexpression of the quadriceps muscle tissue incomparison to continuous training group.