Abstract:
Background. The determination of the genetic endowment of athletic performance in sports is an important step in developing personal training sessions or nutritional supplements for success in sports. Information about the genetic parameters responsible for these metabolisms will help sport’s scientist to develop new insights for better performance. Muscle metabolism is one of the key points in better personal athletic performance.
Objectives. The aim of this study is to analyze the distribution of the methylenetetrahydrofolate reductase enzyme (MTHFR) rs1801133 (C677T) genotype and allele distribution in a Turkish professional cyclist cohort.
Methods. There were 25 Turkish cyclists enrolled in the study. Peripheral blood used for DNA isolation and the conventional polymerase chain reaction- restriction fragment length polymorphism (PCR- RFLP) methodology were used for genotyping.
Results. There were 14 (56%), 10 (40%) and 1 (4%) cyclist who had CC, CT, and TT genotypes, respectively. C allele was counted as 38 (76%), and T alleles as 12 (24%). 9 (50%) of the male cyclist had CC, 8 (44.4%) had CT and only 1 had TT (5.6%) genotypes. C allele was counted as 26 (72.2%), and the T allele as 10 (27.8%) in the male cyclists. In the females, the respective genotypes for CC and CT were 5 (71.4%) and 2 (28.6%). C allele was counted as 12 (85.7%) and T allele as 2 (14.3%).
Conclusion. In our cohort, both of the two genders, the CC genotype and C allele were found to be higher when compared to the other genotypes and T allele. Larger prospective studies focusing on the influence of MTHFR rs1801133 polymorphism in athletic performance are required for confirmation of our findings
Machine summary:
The determination of the genetic endowment of athletic performance in sports is an important step indeveloping personal training sessions or nutritional supplements for success in sports.
The aim of this studyis to analyze the distribution of the methylenetetrahydrofolate reductase enzyme (MTHFR) rs1801133 (C677T)genotype and allele distribution in a Turkish professional cyclist cohort.
Distribution of Methylenetetrahydrofolate Reductase rs1801133Polymorphism in a Turkish Professional Cyclist Cohort1İpek Yüksel, 1Canan Sercan, 1Sezgin Kapıcı, 1Betül Bıyık, 1Tugba Kaman, 1,2Korkut Ulucan*1Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University,Istanbul, Turkey.
As reducedenzyme activity disturbs DNA methylation byeffecting regulatory the mechanismscontrolling genes involved in musclehypertrophy, we aimed to analyze MTHFRC677T rs1801133 polymorphism inprofessional Turkish cyclists.
The genotype and allelic distributionsfor the MTHFR gene are summarized in Table 1.
The numbers and percentages of the MTHFR rs1801133 genotype and alleles in the cyclists.
MTHFR Genotype Allele FrequencyCC CT TT C TFemale Cyclists 5 (71.
In their cohort, they identifieda significant difference between MTHFRrs1801133 polymorphic alleles and the analyzedparameters; the soccer players were found to havelower levels of triglyceride, total cholesterol andLDL cholesterol levels, and higher levels of folatethan the sedentary individuals.
A Russian cohort from the same studyfor endurance athletes showed similar results toours; their respective genotype percentages forCC, CT, and TT were 51, 43 and 6.
Genetic polymorphisms of the enzymesinvolved in DNA methylation and synthesis in elite athletes.
The effect of the MTHFR C677T mutation on athletic performance andthe homocysteine level of soccer players and sedentary individuals.